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fastking rt kit with gdnase  (tiangen biotech co)


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    Structured Review

    tiangen biotech co fastking rt kit with gdnase
    Fastking Rt Kit With Gdnase, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 99/100, based on 660 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fastking/FastKing+RT+Kit/pmc13010918-85-18-23
    Average 99 stars, based on 660 article reviews
    fastking rt kit with gdnase - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Expressing:

    Article Title: Profiles of Intestinal Flora in Breastfed Obese Children and Selecting Functional Strains Against Obesity.
    Article Snippet: Scope: Breast milk has the potential to prevent childhood obesity by providing probiotics, but there are still instances of obesity in breastfed children.. Methods and Results: This study investigates the difference in intestinal flora structure between breastfed children with obesity (OB-BF) and normal-weight breastfed children (N-BF).. Building upon this foundation, it employs both cell and mouse models to identify an antiobesity strain within the fecal matter of N-BF children and explore its underlying mechanisms.

    Article Title: Glutathione peroxidase gene regulates substrate development and prevents strain aging in Volvariella volvacea.
    Article Snippet: Degradation of Volvariella volvacea is associated with the accumulation of reactive oxygen species (ROS), and glutathione peroxidase (GPX) is one of the key antioxidant enzyme.. The purpose of this research is to uncover the importance of the gpx gene in the degradation and revitalization of V. volvacea.. In this study, a gpx-silenced strain of the primordial strain T0 and an overexpression strain of the degraded strain T19 of V. volvacea were constructed, and their antioxidant properties, matrix degradation ability, and production traits were determined.

    Article Title: Narirutin downregulates USP15 to inhibit the NLRP3 ubiquitination in HDM-induced allergic rhinitis
    Article Snippet: 1 Abbreviations: Nar, Nariruitin; USP15, ubiquitin-specific peptidase 15; HDM, house dust mite; PAS, Periodic acidSchiff; H&E, hematoxylin and eosin; BALF, bronchoalveolar lavage fluid; AR, allergic rhinitis; HNEpCs, Human Nasal Epithelial Cells; LPS, Lipopolysaccharide; ATP, Adenosine TriPhosphate; mtSOX, Mitochondrial Superoxide; mtTracker,

    Reverse Transcription:

    Article Title: Profiles of Intestinal Flora in Breastfed Obese Children and Selecting Functional Strains Against Obesity.
    Article Snippet: Scope: Breast milk has the potential to prevent childhood obesity by providing probiotics, but there are still instances of obesity in breastfed children.. Methods and Results: This study investigates the difference in intestinal flora structure between breastfed children with obesity (OB-BF) and normal-weight breastfed children (N-BF).. Building upon this foundation, it employs both cell and mouse models to identify an antiobesity strain within the fecal matter of N-BF children and explore its underlying mechanisms.

    Article Title: Glutathione peroxidase gene regulates substrate development and prevents strain aging in Volvariella volvacea.
    Article Snippet: Degradation of Volvariella volvacea is associated with the accumulation of reactive oxygen species (ROS), and glutathione peroxidase (GPX) is one of the key antioxidant enzyme.. The purpose of this research is to uncover the importance of the gpx gene in the degradation and revitalization of V. volvacea.. In this study, a gpx-silenced strain of the primordial strain T0 and an overexpression strain of the degraded strain T19 of V. volvacea were constructed, and their antioxidant properties, matrix degradation ability, and production traits were determined.

    Article Title: Role of Th2, Th17 and Treg Cells and relevant cytokines in pathogenesis of allergic rhinitis
    Article Snippet: Total RNA was extracted from mononuclear cells according to the instructions of the total RNA extraction kit of Tiangen Biotech (Beijing) Co., Ltd. After extraction, the concentration and purity of RNA were determined using an ultraviolet (UV) spectrophotometer, and then the RNA samples were cryopreserved in a refrigerator at -80°C for next use. .. With total RNA of mononuclear cells as template and GAPDH as internal reference gene (forward primer: 5’CAAGGCTGTGGGCAAGGTCATC-3’, reverse primer: 5’-GTGTCGCTGTTGAAGTCAGAGGAG-3’), the reaction system was added according to the instructions of the FastKing one-step RT-PCR kit produced by Tiangen Biotech (Beijing) Co., Ltd. for reverse transcription (42°C 30 min, 95°C 3 min) and RT-PCR (94°C 30 s, 65°C 30s, 72°C 30 s, 40 cycles, 72°C 5 min). .. ABI 7500 real-time fluorescent quantitative PCR instrument was used for detecting the levels of GATA-3 mRNA (forward primer: 5’-CAGTTGGCCTAAGGTGGTT-3’, reverse primer: 5’-GCACGCTGGTAGCTCATACA-3’), RORγt mRNA (forward primer: 5’-GCTGTGATCTTGCCCAGAACC-3’, reverse primer: 5’-CTGCCCATCATTGCTGTTAATCC-3’), and FoxP3 mRNA (forward primer: 5’-CTGCCCCTAGTCATGGTGG-3’, reverse primer: 5’-CTGGAGGAGTGCCTGTAAGTG-3’).

    Article Title: TMEM41B is an endoplasmic reticulum Ca 2+ release channel maintaining naive T cell quiescence and responsiveness
    Article Snippet: .. Total RNA from sorted naive T cells was extracted using the RNA Easy Fast animal tissues/cells total RNA extraction kit (TIANGEN, Cat# DP451) following the manufacturer’s protocol. cDNA library was prepared by reverse transcription using FastKing one-step RT-PCR kit (TIANGEN, Cat# KR123) and further amplified by Taq SYBR® Green qPCR premix (LABLEAD, Cat# R0202) at CFX96 Real-Time PCR System (Bio-Rad). qPCR primers used in this study were: Cd25 -Forward sequence: GCGTTGCTTAGGAAACTCCTGG; Cd25 -Reverse sequence: GCATAGACTGTGTTGGCTTCTGC; Cd132 -Forward sequence: GGAGCAACAGAGATCGAAGCTG; Cd132 -Reverse sequence: CCACAGATTGGGTTATAGCGGC; Cd127 -Forward sequence: CACAGCCAGTTGGAAGTGGATG; Cd127 -Reverse sequence: GGCATTTCACTCGTAAAAGAGCC; Jun -Forward sequence: CAGTCCAGCAATGGGCACATCA; Jun -Reverse sequence: GGAAGCGTGTTCTGGCTATGCA; Junb -Forward sequence: GACCTGCACAAGATGAACCACG; Junb -Reverse sequence: ACTGCTGAGGTTGGTGTAGACG; Myc -Forward sequence: TCGCTGCTGTCCTCCGAGTCC; Myc -Reverse sequence: GGTTTGCCTCTTCTCCACAGAC; Hprt -Forward sequence: CTGGTGAAAAGGACCTCTCGAAG; Hprt -Reverse sequence: CCAGTTTCACTAATGACACAAACG. ..

    SYBR Green Assay:

    Article Title: Profiles of Intestinal Flora in Breastfed Obese Children and Selecting Functional Strains Against Obesity.
    Article Snippet: Scope: Breast milk has the potential to prevent childhood obesity by providing probiotics, but there are still instances of obesity in breastfed children.. Methods and Results: This study investigates the difference in intestinal flora structure between breastfed children with obesity (OB-BF) and normal-weight breastfed children (N-BF).. Building upon this foundation, it employs both cell and mouse models to identify an antiobesity strain within the fecal matter of N-BF children and explore its underlying mechanisms.

    Article Title: TMEM41B is an endoplasmic reticulum Ca 2+ release channel maintaining naive T cell quiescence and responsiveness
    Article Snippet: .. Total RNA from sorted naive T cells was extracted using the RNA Easy Fast animal tissues/cells total RNA extraction kit (TIANGEN, Cat# DP451) following the manufacturer’s protocol. cDNA library was prepared by reverse transcription using FastKing one-step RT-PCR kit (TIANGEN, Cat# KR123) and further amplified by Taq SYBR® Green qPCR premix (LABLEAD, Cat# R0202) at CFX96 Real-Time PCR System (Bio-Rad). qPCR primers used in this study were: Cd25 -Forward sequence: GCGTTGCTTAGGAAACTCCTGG; Cd25 -Reverse sequence: GCATAGACTGTGTTGGCTTCTGC; Cd132 -Forward sequence: GGAGCAACAGAGATCGAAGCTG; Cd132 -Reverse sequence: CCACAGATTGGGTTATAGCGGC; Cd127 -Forward sequence: CACAGCCAGTTGGAAGTGGATG; Cd127 -Reverse sequence: GGCATTTCACTCGTAAAAGAGCC; Jun -Forward sequence: CAGTCCAGCAATGGGCACATCA; Jun -Reverse sequence: GGAAGCGTGTTCTGGCTATGCA; Junb -Forward sequence: GACCTGCACAAGATGAACCACG; Junb -Reverse sequence: ACTGCTGAGGTTGGTGTAGACG; Myc -Forward sequence: TCGCTGCTGTCCTCCGAGTCC; Myc -Reverse sequence: GGTTTGCCTCTTCTCCACAGAC; Hprt -Forward sequence: CTGGTGAAAAGGACCTCTCGAAG; Hprt -Reverse sequence: CCAGTTTCACTAATGACACAAACG. ..

    Over Expression:

    Article Title: Glutathione peroxidase gene regulates substrate development and prevents strain aging in Volvariella volvacea.
    Article Snippet: Degradation of Volvariella volvacea is associated with the accumulation of reactive oxygen species (ROS), and glutathione peroxidase (GPX) is one of the key antioxidant enzyme.. The purpose of this research is to uncover the importance of the gpx gene in the degradation and revitalization of V. volvacea.. In this study, a gpx-silenced strain of the primordial strain T0 and an overexpression strain of the degraded strain T19 of V. volvacea were constructed, and their antioxidant properties, matrix degradation ability, and production traits were determined.

    Control:

    Article Title: Glutathione peroxidase gene regulates substrate development and prevents strain aging in Volvariella volvacea.
    Article Snippet: Degradation of Volvariella volvacea is associated with the accumulation of reactive oxygen species (ROS), and glutathione peroxidase (GPX) is one of the key antioxidant enzyme.. The purpose of this research is to uncover the importance of the gpx gene in the degradation and revitalization of V. volvacea.. In this study, a gpx-silenced strain of the primordial strain T0 and an overexpression strain of the degraded strain T19 of V. volvacea were constructed, and their antioxidant properties, matrix degradation ability, and production traits were determined.

    Gene Expression:

    Article Title: Glutathione peroxidase gene regulates substrate development and prevents strain aging in Volvariella volvacea.
    Article Snippet: Degradation of Volvariella volvacea is associated with the accumulation of reactive oxygen species (ROS), and glutathione peroxidase (GPX) is one of the key antioxidant enzyme.. The purpose of this research is to uncover the importance of the gpx gene in the degradation and revitalization of V. volvacea.. In this study, a gpx-silenced strain of the primordial strain T0 and an overexpression strain of the degraded strain T19 of V. volvacea were constructed, and their antioxidant properties, matrix degradation ability, and production traits were determined.

    One Step RT-PCR:

    Article Title: Glutathione peroxidase gene regulates substrate development and prevents strain aging in Volvariella volvacea.
    Article Snippet: Degradation of Volvariella volvacea is associated with the accumulation of reactive oxygen species (ROS), and glutathione peroxidase (GPX) is one of the key antioxidant enzyme.. The purpose of this research is to uncover the importance of the gpx gene in the degradation and revitalization of V. volvacea.. In this study, a gpx-silenced strain of the primordial strain T0 and an overexpression strain of the degraded strain T19 of V. volvacea were constructed, and their antioxidant properties, matrix degradation ability, and production traits were determined.

    Article Title: AURKC Promotes Clear Cell Renal Cell Carcinoma Proliferation Through Upregulation of ERp57
    Article Snippet: .. The FastKing One-Step RT-PCR Kit (code KR123; Tiangen Biochemical Technology Co., Ltd., China) was then utilized, incorporating specific primers into the cDNA template. ..

    Article Title: Role of Th2, Th17 and Treg Cells and relevant cytokines in pathogenesis of allergic rhinitis
    Article Snippet: Total RNA was extracted from mononuclear cells according to the instructions of the total RNA extraction kit of Tiangen Biotech (Beijing) Co., Ltd. After extraction, the concentration and purity of RNA were determined using an ultraviolet (UV) spectrophotometer, and then the RNA samples were cryopreserved in a refrigerator at -80°C for next use. .. With total RNA of mononuclear cells as template and GAPDH as internal reference gene (forward primer: 5’CAAGGCTGTGGGCAAGGTCATC-3’, reverse primer: 5’-GTGTCGCTGTTGAAGTCAGAGGAG-3’), the reaction system was added according to the instructions of the FastKing one-step RT-PCR kit produced by Tiangen Biotech (Beijing) Co., Ltd. for reverse transcription (42°C 30 min, 95°C 3 min) and RT-PCR (94°C 30 s, 65°C 30s, 72°C 30 s, 40 cycles, 72°C 5 min). .. ABI 7500 real-time fluorescent quantitative PCR instrument was used for detecting the levels of GATA-3 mRNA (forward primer: 5’-CAGTTGGCCTAAGGTGGTT-3’, reverse primer: 5’-GCACGCTGGTAGCTCATACA-3’), RORγt mRNA (forward primer: 5’-GCTGTGATCTTGCCCAGAACC-3’, reverse primer: 5’-CTGCCCATCATTGCTGTTAATCC-3’), and FoxP3 mRNA (forward primer: 5’-CTGCCCCTAGTCATGGTGG-3’, reverse primer: 5’-CTGGAGGAGTGCCTGTAAGTG-3’).

    Article Title: TMEM41B is an endoplasmic reticulum Ca 2+ release channel maintaining naive T cell quiescence and responsiveness
    Article Snippet: .. Total RNA from sorted naive T cells was extracted using the RNA Easy Fast animal tissues/cells total RNA extraction kit (TIANGEN, Cat# DP451) following the manufacturer’s protocol. cDNA library was prepared by reverse transcription using FastKing one-step RT-PCR kit (TIANGEN, Cat# KR123) and further amplified by Taq SYBR® Green qPCR premix (LABLEAD, Cat# R0202) at CFX96 Real-Time PCR System (Bio-Rad). qPCR primers used in this study were: Cd25 -Forward sequence: GCGTTGCTTAGGAAACTCCTGG; Cd25 -Reverse sequence: GCATAGACTGTGTTGGCTTCTGC; Cd132 -Forward sequence: GGAGCAACAGAGATCGAAGCTG; Cd132 -Reverse sequence: CCACAGATTGGGTTATAGCGGC; Cd127 -Forward sequence: CACAGCCAGTTGGAAGTGGATG; Cd127 -Reverse sequence: GGCATTTCACTCGTAAAAGAGCC; Jun -Forward sequence: CAGTCCAGCAATGGGCACATCA; Jun -Reverse sequence: GGAAGCGTGTTCTGGCTATGCA; Junb -Forward sequence: GACCTGCACAAGATGAACCACG; Junb -Reverse sequence: ACTGCTGAGGTTGGTGTAGACG; Myc -Forward sequence: TCGCTGCTGTCCTCCGAGTCC; Myc -Reverse sequence: GGTTTGCCTCTTCTCCACAGAC; Hprt -Forward sequence: CTGGTGAAAAGGACCTCTCGAAG; Hprt -Reverse sequence: CCAGTTTCACTAATGACACAAACG. ..

    Article Title: Single-Atom Ce-N-C Nanozyme Ameliorates Type 2 Diabetes Mellitus by Improving Glucose Metabolism Disorders and Reducing Oxidative Stress
    Article Snippet: Total RNA was extracted from liver tissues or HepG2 cells using Trizol (15596018, Ambion, Austin, TX, USA) and quantified using a NanoDropTM spectrophotometer (Thermo Scientific, Waltham, MA, USA), and cDNA was synthesized using a kit (AT311, TransGen Biotech, Beijing, China). .. The mRNA levels of the target genes were then detected using the FastKing One-Step RT-PCR Kit. (FP 205, TIANGEN Biotech, Beijing, China). ..

    Quantitative RT-PCR:

    Article Title: Glutathione peroxidase gene regulates substrate development and prevents strain aging in Volvariella volvacea.
    Article Snippet: Degradation of Volvariella volvacea is associated with the accumulation of reactive oxygen species (ROS), and glutathione peroxidase (GPX) is one of the key antioxidant enzyme.. The purpose of this research is to uncover the importance of the gpx gene in the degradation and revitalization of V. volvacea.. In this study, a gpx-silenced strain of the primordial strain T0 and an overexpression strain of the degraded strain T19 of V. volvacea were constructed, and their antioxidant properties, matrix degradation ability, and production traits were determined.

    Produced:

    Article Title: Role of Th2, Th17 and Treg Cells and relevant cytokines in pathogenesis of allergic rhinitis
    Article Snippet: Total RNA was extracted from mononuclear cells according to the instructions of the total RNA extraction kit of Tiangen Biotech (Beijing) Co., Ltd. After extraction, the concentration and purity of RNA were determined using an ultraviolet (UV) spectrophotometer, and then the RNA samples were cryopreserved in a refrigerator at -80°C for next use. .. With total RNA of mononuclear cells as template and GAPDH as internal reference gene (forward primer: 5’CAAGGCTGTGGGCAAGGTCATC-3’, reverse primer: 5’-GTGTCGCTGTTGAAGTCAGAGGAG-3’), the reaction system was added according to the instructions of the FastKing one-step RT-PCR kit produced by Tiangen Biotech (Beijing) Co., Ltd. for reverse transcription (42°C 30 min, 95°C 3 min) and RT-PCR (94°C 30 s, 65°C 30s, 72°C 30 s, 40 cycles, 72°C 5 min). .. ABI 7500 real-time fluorescent quantitative PCR instrument was used for detecting the levels of GATA-3 mRNA (forward primer: 5’-CAGTTGGCCTAAGGTGGTT-3’, reverse primer: 5’-GCACGCTGGTAGCTCATACA-3’), RORγt mRNA (forward primer: 5’-GCTGTGATCTTGCCCAGAACC-3’, reverse primer: 5’-CTGCCCATCATTGCTGTTAATCC-3’), and FoxP3 mRNA (forward primer: 5’-CTGCCCCTAGTCATGGTGG-3’, reverse primer: 5’-CTGGAGGAGTGCCTGTAAGTG-3’).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Role of Th2, Th17 and Treg Cells and relevant cytokines in pathogenesis of allergic rhinitis
    Article Snippet: Total RNA was extracted from mononuclear cells according to the instructions of the total RNA extraction kit of Tiangen Biotech (Beijing) Co., Ltd. After extraction, the concentration and purity of RNA were determined using an ultraviolet (UV) spectrophotometer, and then the RNA samples were cryopreserved in a refrigerator at -80°C for next use. .. With total RNA of mononuclear cells as template and GAPDH as internal reference gene (forward primer: 5’CAAGGCTGTGGGCAAGGTCATC-3’, reverse primer: 5’-GTGTCGCTGTTGAAGTCAGAGGAG-3’), the reaction system was added according to the instructions of the FastKing one-step RT-PCR kit produced by Tiangen Biotech (Beijing) Co., Ltd. for reverse transcription (42°C 30 min, 95°C 3 min) and RT-PCR (94°C 30 s, 65°C 30s, 72°C 30 s, 40 cycles, 72°C 5 min). .. ABI 7500 real-time fluorescent quantitative PCR instrument was used for detecting the levels of GATA-3 mRNA (forward primer: 5’-CAGTTGGCCTAAGGTGGTT-3’, reverse primer: 5’-GCACGCTGGTAGCTCATACA-3’), RORγt mRNA (forward primer: 5’-GCTGTGATCTTGCCCAGAACC-3’, reverse primer: 5’-CTGCCCATCATTGCTGTTAATCC-3’), and FoxP3 mRNA (forward primer: 5’-CTGCCCCTAGTCATGGTGG-3’, reverse primer: 5’-CTGGAGGAGTGCCTGTAAGTG-3’).

    Synthesized:

    Article Title: Inheritance and ecological effects of exogenous genes from transgenic Brassica napus to Brassica juncea hybrids.
    Article Snippet: ● With the rapid development of new breeding techniques, the ecological impacts of transgenic plants receive wide concern again, particularly for potential gene flow from transgenic crops to their relatives.. The transgene insertion position, number of gene copies, and flanking sequence of exogenous genes integrated into the recipient genome affect the genetic stability and fitness of offspring.. ● We employed hybrids F1 and F2, six backcross generations BC1-BC6 and BC1F1 from transgenic Brassica napus with Bacillus thuringiensis (Bt) cry1Ac gene and its wild relative B. juncea through hand pollination.

    RNA Extraction:

    Article Title: TMEM41B is an endoplasmic reticulum Ca 2+ release channel maintaining naive T cell quiescence and responsiveness
    Article Snippet: .. Total RNA from sorted naive T cells was extracted using the RNA Easy Fast animal tissues/cells total RNA extraction kit (TIANGEN, Cat# DP451) following the manufacturer’s protocol. cDNA library was prepared by reverse transcription using FastKing one-step RT-PCR kit (TIANGEN, Cat# KR123) and further amplified by Taq SYBR® Green qPCR premix (LABLEAD, Cat# R0202) at CFX96 Real-Time PCR System (Bio-Rad). qPCR primers used in this study were: Cd25 -Forward sequence: GCGTTGCTTAGGAAACTCCTGG; Cd25 -Reverse sequence: GCATAGACTGTGTTGGCTTCTGC; Cd132 -Forward sequence: GGAGCAACAGAGATCGAAGCTG; Cd132 -Reverse sequence: CCACAGATTGGGTTATAGCGGC; Cd127 -Forward sequence: CACAGCCAGTTGGAAGTGGATG; Cd127 -Reverse sequence: GGCATTTCACTCGTAAAAGAGCC; Jun -Forward sequence: CAGTCCAGCAATGGGCACATCA; Jun -Reverse sequence: GGAAGCGTGTTCTGGCTATGCA; Junb -Forward sequence: GACCTGCACAAGATGAACCACG; Junb -Reverse sequence: ACTGCTGAGGTTGGTGTAGACG; Myc -Forward sequence: TCGCTGCTGTCCTCCGAGTCC; Myc -Reverse sequence: GGTTTGCCTCTTCTCCACAGAC; Hprt -Forward sequence: CTGGTGAAAAGGACCTCTCGAAG; Hprt -Reverse sequence: CCAGTTTCACTAATGACACAAACG. ..

    cDNA Library Assay:

    Article Title: TMEM41B is an endoplasmic reticulum Ca 2+ release channel maintaining naive T cell quiescence and responsiveness
    Article Snippet: .. Total RNA from sorted naive T cells was extracted using the RNA Easy Fast animal tissues/cells total RNA extraction kit (TIANGEN, Cat# DP451) following the manufacturer’s protocol. cDNA library was prepared by reverse transcription using FastKing one-step RT-PCR kit (TIANGEN, Cat# KR123) and further amplified by Taq SYBR® Green qPCR premix (LABLEAD, Cat# R0202) at CFX96 Real-Time PCR System (Bio-Rad). qPCR primers used in this study were: Cd25 -Forward sequence: GCGTTGCTTAGGAAACTCCTGG; Cd25 -Reverse sequence: GCATAGACTGTGTTGGCTTCTGC; Cd132 -Forward sequence: GGAGCAACAGAGATCGAAGCTG; Cd132 -Reverse sequence: CCACAGATTGGGTTATAGCGGC; Cd127 -Forward sequence: CACAGCCAGTTGGAAGTGGATG; Cd127 -Reverse sequence: GGCATTTCACTCGTAAAAGAGCC; Jun -Forward sequence: CAGTCCAGCAATGGGCACATCA; Jun -Reverse sequence: GGAAGCGTGTTCTGGCTATGCA; Junb -Forward sequence: GACCTGCACAAGATGAACCACG; Junb -Reverse sequence: ACTGCTGAGGTTGGTGTAGACG; Myc -Forward sequence: TCGCTGCTGTCCTCCGAGTCC; Myc -Reverse sequence: GGTTTGCCTCTTCTCCACAGAC; Hprt -Forward sequence: CTGGTGAAAAGGACCTCTCGAAG; Hprt -Reverse sequence: CCAGTTTCACTAATGACACAAACG. ..

    Amplification:

    Article Title: TMEM41B is an endoplasmic reticulum Ca 2+ release channel maintaining naive T cell quiescence and responsiveness
    Article Snippet: .. Total RNA from sorted naive T cells was extracted using the RNA Easy Fast animal tissues/cells total RNA extraction kit (TIANGEN, Cat# DP451) following the manufacturer’s protocol. cDNA library was prepared by reverse transcription using FastKing one-step RT-PCR kit (TIANGEN, Cat# KR123) and further amplified by Taq SYBR® Green qPCR premix (LABLEAD, Cat# R0202) at CFX96 Real-Time PCR System (Bio-Rad). qPCR primers used in this study were: Cd25 -Forward sequence: GCGTTGCTTAGGAAACTCCTGG; Cd25 -Reverse sequence: GCATAGACTGTGTTGGCTTCTGC; Cd132 -Forward sequence: GGAGCAACAGAGATCGAAGCTG; Cd132 -Reverse sequence: CCACAGATTGGGTTATAGCGGC; Cd127 -Forward sequence: CACAGCCAGTTGGAAGTGGATG; Cd127 -Reverse sequence: GGCATTTCACTCGTAAAAGAGCC; Jun -Forward sequence: CAGTCCAGCAATGGGCACATCA; Jun -Reverse sequence: GGAAGCGTGTTCTGGCTATGCA; Junb -Forward sequence: GACCTGCACAAGATGAACCACG; Junb -Reverse sequence: ACTGCTGAGGTTGGTGTAGACG; Myc -Forward sequence: TCGCTGCTGTCCTCCGAGTCC; Myc -Reverse sequence: GGTTTGCCTCTTCTCCACAGAC; Hprt -Forward sequence: CTGGTGAAAAGGACCTCTCGAAG; Hprt -Reverse sequence: CCAGTTTCACTAATGACACAAACG. ..

    Real-time Polymerase Chain Reaction:

    Article Title: TMEM41B is an endoplasmic reticulum Ca 2+ release channel maintaining naive T cell quiescence and responsiveness
    Article Snippet: .. Total RNA from sorted naive T cells was extracted using the RNA Easy Fast animal tissues/cells total RNA extraction kit (TIANGEN, Cat# DP451) following the manufacturer’s protocol. cDNA library was prepared by reverse transcription using FastKing one-step RT-PCR kit (TIANGEN, Cat# KR123) and further amplified by Taq SYBR® Green qPCR premix (LABLEAD, Cat# R0202) at CFX96 Real-Time PCR System (Bio-Rad). qPCR primers used in this study were: Cd25 -Forward sequence: GCGTTGCTTAGGAAACTCCTGG; Cd25 -Reverse sequence: GCATAGACTGTGTTGGCTTCTGC; Cd132 -Forward sequence: GGAGCAACAGAGATCGAAGCTG; Cd132 -Reverse sequence: CCACAGATTGGGTTATAGCGGC; Cd127 -Forward sequence: CACAGCCAGTTGGAAGTGGATG; Cd127 -Reverse sequence: GGCATTTCACTCGTAAAAGAGCC; Jun -Forward sequence: CAGTCCAGCAATGGGCACATCA; Jun -Reverse sequence: GGAAGCGTGTTCTGGCTATGCA; Junb -Forward sequence: GACCTGCACAAGATGAACCACG; Junb -Reverse sequence: ACTGCTGAGGTTGGTGTAGACG; Myc -Forward sequence: TCGCTGCTGTCCTCCGAGTCC; Myc -Reverse sequence: GGTTTGCCTCTTCTCCACAGAC; Hprt -Forward sequence: CTGGTGAAAAGGACCTCTCGAAG; Hprt -Reverse sequence: CCAGTTTCACTAATGACACAAACG. ..

    Sequencing:

    Article Title: TMEM41B is an endoplasmic reticulum Ca 2+ release channel maintaining naive T cell quiescence and responsiveness
    Article Snippet: .. Total RNA from sorted naive T cells was extracted using the RNA Easy Fast animal tissues/cells total RNA extraction kit (TIANGEN, Cat# DP451) following the manufacturer’s protocol. cDNA library was prepared by reverse transcription using FastKing one-step RT-PCR kit (TIANGEN, Cat# KR123) and further amplified by Taq SYBR® Green qPCR premix (LABLEAD, Cat# R0202) at CFX96 Real-Time PCR System (Bio-Rad). qPCR primers used in this study were: Cd25 -Forward sequence: GCGTTGCTTAGGAAACTCCTGG; Cd25 -Reverse sequence: GCATAGACTGTGTTGGCTTCTGC; Cd132 -Forward sequence: GGAGCAACAGAGATCGAAGCTG; Cd132 -Reverse sequence: CCACAGATTGGGTTATAGCGGC; Cd127 -Forward sequence: CACAGCCAGTTGGAAGTGGATG; Cd127 -Reverse sequence: GGCATTTCACTCGTAAAAGAGCC; Jun -Forward sequence: CAGTCCAGCAATGGGCACATCA; Jun -Reverse sequence: GGAAGCGTGTTCTGGCTATGCA; Junb -Forward sequence: GACCTGCACAAGATGAACCACG; Junb -Reverse sequence: ACTGCTGAGGTTGGTGTAGACG; Myc -Forward sequence: TCGCTGCTGTCCTCCGAGTCC; Myc -Reverse sequence: GGTTTGCCTCTTCTCCACAGAC; Hprt -Forward sequence: CTGGTGAAAAGGACCTCTCGAAG; Hprt -Reverse sequence: CCAGTTTCACTAATGACACAAACG. ..



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    tiangen biotech co cdna first strand synthesis kit
    Cdna First Strand Synthesis Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fastking/FastKing+gDNA+Dispelling+RT+SuperMix/10__1096_slash_fj__202503248rr-101-6-10
    Average 96 stars, based on 1 article reviews
    cdna first strand synthesis kit - by Bioz Stars, 2026-10
    96/100 stars
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